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分類清單
張崇德
 張崇德老師
職稱 助理教授
學歷 PhD, Molecular Biology and Biotechnology, University of Sheffield, UK
經歷 Postdoctoral fellow, Max Planck Institute for Developmental Biology, Germany
辦公室 生物醫學大樓 五樓 R505 室
電話 886-2-28267122
Email chungte.chang@ym.edu.tw
專長--基因轉錄後調控, 5'-3' mRNA降解機制, miRNA調控機制
研究方向

在過去,基因表達(gene expression)被認為主要是受到轉錄水平(transcription level)的調節。然而,由於RNA沉默(RNA silencing)途徑的發現,以及在轉錄後調控(Post-transcriptional regulation)領域的研究,使人們認識到轉錄後調控機制提供了細胞得以迅速改變基因表達的模式。在基因轉錄後的過程中(例如: mRNA processing,export,surveillance,silencing和turnover),經由通過使用共同因子的相互連結,構成了極為複雜的調節網絡,其有助於細胞和生物體特異性基因的表達及面對外在環境的快速應變能力。

為了能進一步了解基因轉錄後水平調節表達的分子機制,我們的長期目標是:

  • 1.為轉錄後途徑建立一個綜合的效應子和相互作用的網絡列表,以了解這些途徑是如何相互聯繫及調控;
  • 2.了解這些轉錄後過程對整體基因表達的貢獻,並確定內源性靶標;
  • 3.解析當這些途徑被擾動時,內源性靶標的調節如何導致在細胞和生物體中觀察到的複雜表型。
研究著作

Chang CT, Muthukumar S, Weber R, Levdansky Y, Chen Y, Bhandari D, Igreja C, Wohlbold L, Valkov E, Izaurralde E. (2019) A low-complexity region in human XRN1 directly recruits deadenylation and decapping factors in 5'-3' messenger RNA decay. Nucleic Acids Res. [Epub ahead of print]

 

Raisch T*, Chang CT*, Levdansky Y*, Muthukumar S, Raunser S, Valkov E. (2019) Reconstitution of recombinant human CCR4-NOT reveals molecular insights into regulated deadenylation. Nat Commun. 10:3173. (*co-first)

 

Valkov E*, Muthukumar S*, Chang CT*, Jonas S, Weichenrieder O, Izaurralde E. (2016) Structure of the Dcp2-Dcp1 mRNA-decapping complex in the activated conformation. Nat Struct Mol Biol. 23:574-9. (*co-first)

 

Chen Y, Boland A, Kuzuoğlu-Öztürk D, Bawankar P, Loh B, Chang CT, Weichenrieder O, Izaurralde E. (2014) A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct links between miRNA target recognition and silencing. Mol Cell. 54:737-50.

 

Chang CT, Bercovich N, Loh B, Jonas S, Izaurralde E. (2014) The activation of the decapping enzyme DCP2 by DCP1 occurs on the EDC4 scaffold and involves a conserved loop in DCP1. Nucleic Acids Res. 42:5217-33.

 

Chang CT*, Hautbergue GM*, Walsh MJ, Viphakone N, van Dijk TB, Philipsen S, Wilson SA. (2013) Chtop is a component of the dynamic TREX mRNA export complex. EMBO J. 32:473-86. (*co-first)

 

Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, Weichenrieder O, Coles M, Izaurralde E. (2012) A direct interaction between DCP1 and XRN1 couples mRNA decapping to 5' exonucleolytic degradation. Nat Struct Mol Biol. 19:1324-31.

 

Viphakone N, Hautbergue GM, Walsh M, Chang CT, Holland A, Folco EG, Reed R, Wilson SA. (2012) TREX exposes the RNA-binding domain of Nxf1 to enable mRNA export. Nat Commun. 3:1006.

 

Cruz-Migoni A, Hautbergue GM, Artymiuk PJ, Baker PJ, Bokori-Brown M, Chang CT, Dickman MJ, Essex-Lopresti A, Harding SV, Mahadi NM, Marshall LE, Mobbs GW, Mohamed R, Nathan S, Ngugi SA, Ong C, Ooi WF, Partridge LJ, Phillips HL, Raih MF, Ruzheinikov S, Sarkar-Tyson M, Sedelnikova SE, Smither SJ, Tan P, Titball RW, Wilson SA, Rice DW. (2011) A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. Science. 334:821-4.

 

Hautbergue GM, Hung ML, Walsh MJ, Snijders AP, Chang CT, Jones R, Ponting CP, Dickman MJ, Wilson SA. (2009) UIF, a New mRNA export adaptor that works together with REF/ALY, requires FACT for recruitment to mRNA. Curr Biol. 19:1918-24.

 

Su MC, Chang CT, Chu CH, Tsai CH, Chang KY. (2005) An atypical RNA pseudoknot stimulator and an upstream attenuation signal for -1 ribosomal frameshifting of SARS coronavirus. Nucleic Acids Res. 33:4265-75.

 

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