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108學年度進階生化與分生實驗課程表

週別

日期

主題

負責老師

負責助教

1

8/5~8/9

First step to work in Bio-Lab

Wk1-I: Lab safety, instruments and biochemical reagents

Wk1-II: Using microbe for molecular biological experiments

   1.Preparation of competent cell

   2.Bacteria transformation

Wk1-III: Result Discussion

曾炳輝

黃介嶸

鄭閔魁

王毓瑄

楊雅茹

許玉璇

林雅雄

朱孝益

徐子涵

2

8/12~8/16

Molecular Cloning

Wk2-I     Restriction enzyme digestion

Wk2-II    DNA agarose gel electrophoresis

Wk2-III   Gel purification of DNA fragments

Wk2-IV   DNA quantification

Wk2-V    Ligation

Wk2-VI  Result Discussion

王琬菁

郭津岑

 

王毓瑄

楊雅茹

許玉璇

3

8/19~8/23

Plasmid DNA preparation, and restrict enzyme digestion

Wk3-I  Plasmid DNA mini-preparation, enzyme digestion and gel electrophoresis

Wk3-II 以QIAprep Spin Miniprep kit進行質體純化並轉形入 Rosetta strain

Wk3-III  Result Discussion

游麗如

鄭子豪

 

徐子涵

朱孝益

 

4

8/26~8/30

Recombinant Protein

Wk4-I: Generation of recombinant protein by using microbe

Wk4-II: Purification of recombinant protein by column chromatography

Wk4-III: Analysis of recombinant protein by gel electrophoresis

Wk4-IV: Result Discussion

陳威儀

姜為中

鄭閔魁

林雅雄

 

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