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國立陽明交通大學 生化暨分子生物研究所

分類清單

陳威儀

陳威儀
職稱 副教授
學歷 PhD. National Defense Medical College (Institute of Life Science)
經歷 Postdoc. Lab of Biochemistry and Molecular Biology, Rockefeller University, USA
辦公室 生物醫學大樓 七樓 R711 室
電話 886-2-2826-7328
Email chenwy@nycu.edu.tw
學經歷
2020迄今 國立陽明大學 生化暨分子生物研究所 副教授
2017迄今   美國洛克菲勒大學 生化實驗室 客座學者
2014-2020 國立陽明大學 生化暨分子生物研究所 助理教授
2014-2017   美國洛克菲勒大學 生化實驗室 兼任學者
2007-2014   美國洛克菲勒大學 博士後研究員
2005-2007 中央研究院 分子生物所 博士後研究員
2000-2005國防醫學院、中研院、國衛院合辦生命科學研究所 博士
榮譽
2019國立陽明大學 生命科學院 教學優良教師
2019, 2020國立陽明大學 醫學系 良師益友導師
2008-2011   美國血癌及淋巴癌基因會 博士後研究學者
2006 國科會 菁英博士後研究獎助
2006 王民寧基金會 博士班優秀論文獎
指導學生獲獎
2020   

指導博士生畢文潔榮獲陽明大學博士班優良論文獎學金 優等獎

2020   

指導碩士生黃瀞瑩榮獲陽明大學碩士班優良論文獎學金 優等獎

2020   

指導碩士生黃瀞瑩榮獲陽明大學生化所尹珣若論文比賽 第一名

2020   

指導醫學系林家瑋獲得科技部大專生研究計畫補助

2020   

指導醫學系林家瑋獲得健康科學文教基金會研究計畫補助 (declined)

2019   

指導博士生畢文潔榮獲第十六屆亞洲基因轉錄大會(於紐西蘭)壁報論文競賽 第一名

2019

指導博士生畢文潔榮獲科技部博士生出席國際會議補助

2019

指導碩士生林信賢獲得陽明大學校級紀念尹珣若先生品學及論文優勝

2019

指導碩士生林信賢獲得陽明大學生化所尹珣若論文比賽第一名

2018

指導醫學系專題生徐恩杰獲得美國斯隆凱特琳醫學中心暑期研修

2018

指導碩士生黃國誠榮獲陽明大學生化所尹珣若論文比賽第三名

專長-- Transcriptional Regulatory Mechanism of B cell Lymphopoiesis and Leukemogenesis
            基因轉錄調控, 表觀基因體,腫瘤生物學, 生化及分子生物學
研究方向
Antibody producing B cells arise from hematopoietic stem cells through a series of developmental steps that involve a hierarchical transcription factor network. Whereas much is known about their direct genetic targets, little is known about their actual mechanism of action through interactions with various coactivators that act on chromatin structure or on the general transcription machinery. In addition, genetic mutations of the involved transcription factors result in loss-of-function or gain-of-function frequently lead to B cell deficiency or malignancy.
Our goal is to uncover the mechanisms by which transcriptional activators/coactivators (including various histone modifiers/remodelers) control the development and leukemogenesis of B-lymphocytes, as well as searching therapeutic targets/approaches for B-cells associated leukemia/lymphoma.
On-going studies will employ various state-of-the-art biomedical technologies that include:
1. biochemically isolating recombinant proteins/ protein complexes from bacteria/insect/mammalian cell cultures for cell-free assays
2. genome-widely profiling (ChIP-seq and RNA-seq) the transcription-related factors (DNA-binding proteins and transcriptional coactivators) and corresponding histone modifications in normal or malignant cells
3. developing the peptidomimetics for potential therapeutic to leukemia/lymphoma treatment

基因轉錄是細胞展現生命現象的重要環節之一,而不正常 的基因轉錄也是造成疾病與癌症的主要原因,所以研究基因轉錄的分子機轉是生命科學中一個基本且重要的課題。過去 40 年的研究中,這個領域已經發現至少需要 100 多種蛋白的參與才能轉錄 DNA 上的基因資訊,但是對於這些轉錄相關蛋白間的各種交互作用則所知甚少; 另外高通量的次世代定序技術也將基因轉錄的研究從以往的單一基因推展到全基因體的層級,這個技術發展幫助瞭解轉錄作用的普遍法則,更重要的是能提供精準醫學的重要依據。

目前,實驗室的研究主題之一是想瞭解轉錄因子 E2A 融合蛋白造成 B 細胞腫瘤的分子機轉,以及所調控的基因組。 我們發現 E2A 融合蛋白能扮演一般轉錄輔因子的功能,促進另一個轉錄因子 RUNX1 的活性而造成血球幹細胞無法分化,這個發現在未來能提供治療B細胞腫瘤的精準醫療策略。 此外,我們的另一個研究方向是基因轉錄作用如何調控肺癌細胞的轉移。利用高通量全基因體DNA 定序的分析法 (ChIP-seq 及 RNA-seq),我們發現在高轉移能力的肺癌細胞會大量表現 CDCP1 膜蛋白,CDCP1 調控癌細胞轉移已經報導於許多文獻中,但 CDCP1 為何會在癌細胞中大量表現則不清楚。透過表觀基因體的分析,我們發 現 CDCP1 的基因區段只有在高轉移的細胞中會形成超級增強子,瞭解CDCP1超級增強子的形成機轉將有助於提供未來精準醫療的策略。
研究著作

Pi W-C, Wang J, Shimada M, Lin JW, Geng H, Lee Y-L, Li D, Wang GG, Roeder RG, Chen W-Y. E2A-PBX1 functions as a coactivator for RUNX1 in acute lymphoblastic leukemia. Blood 2020; 136, 11-23 (IF: 17.543) (Plenary Article)

 

Wang X, Gerber A, Chen W-Y, Roeder RG. Functions of paralogous RNA polymerase III subunits POLR3G and POLR3GL in mouse development. PNAS 2020; (IF: 9.412)

 

Liao C-C, Shankar S, Pi W-C, Chang C-C, Ahmed GR, Chen W-Y, Hsia K-C. Karyopherin Kap114p-mediated trans-repression controls ribosomal gene expression under saline stress. EMBO reports 2020; 21, e48324 (IF: 7.497)

 

Liu CF, Tseng CH, Huang CY, Sun CC, Yang ML, Chen W-Y*, and Yeung L*. Correlation between Higher-Order Aberrations and Visual Acuity Recovery (CoHORT) after Spectacles Treatment for Pediatric Refractive Amblyopia: A Pilot Study Using iDesign Measurement. PLoS One 2020;15, e0229822. (IF: 2.74) *corresponding

 

Shimada M, Chen W-Y, Nakadai T, Onikubo T, Guermah M, Rhodes D, Roeder RG. Gene-Specific H1 Eviction through a Transcriptional Activator→p300→NAP1→H1 Pathway. Molecular Cell 2019; 74, 268-283. (IF: 15.584)

 

Chang CH, Zanini M, Shirvani H, Cheng JS, Yu H, Feng CH, Mercier AL, Hung SY, Forget A, Wang CH, Cigna SM, Lu IL, Chen W-Y, Leboucher S, Wang WJ, Ruat M, Spassky N, Tsai JW, Ayrault O. Atoh1 Controls Primary Cilia Formation to Allow for SHH-Triggered Granule Neuron Progenitor Proliferation. Developmental Cell 2019; 48, 184-199. (IF: 10.092)

 

Chen YL, Hu CM, Hsu JT, Chang CC, Huang TY, Chiang PH, Chen W-Y, Chang YT, Chang MC, Tien YW, Lee EY, Jeng YM, Lee WH. Cellular 5-hydroxylmethylcytosine content determines tumorigenic potential and prognosis of pancreatic ductal adenocarcinoma. American Journal of Cancer Research 2018; 8, 2548-2563. (IF: 5.177)

 

Jishage M, Yu X, Shi Y, Ganesan SJ, Chen W-Y, Sali A, Chait BT, Asturias FJ, Roeder RG. Architecture of Pol II(G) and molecular mechanism of transcription regulation by Gdown1. Nature Structural and Molecular Biology 2018; 25, 859-867. (IF: 11.98)

 

Wu HT, Kuo YC, Hung JJ, Huang CH, Chen W-Y, Chou TY, Chen Y, Chen YJ, Chen YJ, Cheng WC, Teng SC, Wu KJ. K63-polyubiquitinated HAUSP deubiquitinates HIF-1α and dictates H3K56 acetylation promoting hypoxia-induced tumour progression. Nature Communications 2016; 7, 13644. (IF: 12.121)

 

Lu R, Wang P, Parton T, Zhou Y, Chrysovergis K, Rockowitz S, Chen W-Y, Abdel-Wahab O, Wade PA, Zheng D, Wang GG. Epigenetic Perturbations by Arg882-Mutated DNMT3A Potentiate Aberrant Stem Cell Gene-Expression Program and Acute Leukemia Development. Cancer Cell 2016; 30, 92-107. (IF: 26.602)

 

Jiang BH, Chen W-Y, Li HY, Chien Y, Chang WC, Hsieh PC, Wu P, Chen CY, Song HY, Chien CS, Sung YJ, Chiou SH. CHD1L regulated PARP1-driven Pluripotency and Chromatin Remodeling during the Early-stage Cell Reprogramming. Stem Cells 2015; 33, 2961-2972. (IF: 6.022)

 

Geng H, Hurtz C, Lenz KB, Chen Z, Baumjohann D, Thompson S, Goloviznina NA, Chen W-Y, Huan J, LaTocha D, Ballabio E, Xiao G, Lee JW, Deucher A, Qi Z, Park E, Huang C, Nahar R, Kweon SM, Shojaee S, Chan LN, Yu J, Kornblau SM, Bijl JJ, Ye BH, Ansel KM, Paietta E, Melnick A, Hunger SP, Kurre P, Tyner JW, Loh ML, Roeder RG, Druker BJ, Burger JA, Milne TA, Chang BH, Müschen M. Self-enforcing feedback activation between BCL6 and pre-B cell receptor signaling defines a distinct subtype of acute lymphoblastic leukemia. Cancer Cell 2015; 27, 409-425. (IF: 26.602)

 

Cheng KW, Cheng SC, Chen W-Y, Lin MH, Chuang SJ, Cheng IH, Sun CY, Chou CY. Thiopurine analogs and mycophenolic acid synergistically inhibit the papain-like protease of Middle East respiratory syndrome coronavirus. Antiviral Research 2015; 115, 9-16. (IF 4.101)

 

Chen C-H, Chen W-Y, Lin S-F, and Wong RJ. Epithelial–Mesenchymal Transition Enhances Response to Oncolytic Herpesviral Therapy Through Nectin-1. Human Gene Therapy 2014; 25, 539-551. (IF: 4.273)

 

Chen W-Y, Zhang J, Geng H, Du Z, Nakadai T, and Roeder RG. A TAF4 coactivator function for E proteins that involves enhanced TFIID binding. Genes and Development 2013; 27:1596-1609. (IF: 9.527)

 

Tang Z, Chen W-Y, Shimada M, Nguyen UTT, Kim J, Sun XJ, Sengoku T, McGinty RK, Fernandez JP, Muir TW, and Roeder RG. SET1 and p300 synergy, through coupled histone modifications, in transcriptional activation by p53. Cell 2013; 154, 297-310. (IF: 38.637)

 

Sun XJ, Wang Z, Wang L, Jiang Y, Kost N, Soong TD, Chen W-Y, Tang Z, Nakadai T, Elemento O, Fischle W, Melnick A, Patel DJ, Nimer SD, and Roeder RG. A stable transcription factor complex nucleated by oligomeric AML1-ETO controls leukemogenesis. Nature 2013; 500: 93-97. (IF: 42.778)

 

Cai L, Rothbart SB, Lu R, Xu B, Chen W-Y, Tripathy A, Rockowitz S, Zheng D, Patel DJ, Allis CD, Strahl BD, Song J, and Wang GG. An H3K36 methylation engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting. Molecular Cell 2013; 49: 571-582. (IF: 15.584)

 

Geng H, Brennan S, Milne TA, Chen W-Y, Li Y, Hurtz C, Kweon SM, Zickl L, Shojaee S, Neuberg D, Huang C, Biswas D, Xin Y, Racevskis J, Ketterling RP, Luger SM, Lazarus H, Tallman MS, Rowe JM, Litzow MR, Guzman ML, Allis CD, Roeder RG, Muschen M, Paietta E, Elemento O, Melnick AM. Integrative Epigenomic Analysis Identifies Biomarkers and Therapeutic Targets in Adult B-Acute Lymphoblastic Leukemia. Cancer Discovery 2012; 2: 1004-1023. (IF: 29.497)

 

Jiang H, Shukla A, Wang X, Chen W-Y, Bernstein BE, and Roeder RG. Role for Dpy-30 in ES cell-fate specification by regulation of H3K4 methylation within bivalent domains. Cell 2011;144: 513-525. (IF: 38.637)

 

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