Your browser does not support JavaScript!
  • 職稱: 副教授
  • 學歷: PhD. Institute of Molecular Medicine, National Taiwan University, Taiwan
  • 經歷:Research Fellow, National Heart, Lung and Blood Institute (NHLBI), National Institute of Health (NIH), USA
  • 辦公室: 傳醫乙棟 五樓 R515 室
  • 電話: 886-2-2826-7120
  • Email這個 E-mail 地址已經被防止灌水惡意程式保護,您需要啟用 Java Script 才能觀看
  • 研究領域

    Cells sense the environment through their adhesive organelles, focal adhesions (FAs), which are able to transduce appropriate biochemical signals to control numerous critical cellular events, including cell migration, proliferation, differentiation or death. FAs are macromolecular complex that start to form when the central components, integrin receptors, are activated by engagement with the extracellular matrix (ECM), and recruit a series of FA-associated proteins to connect with actin cytoskeleton. The protein composition in FAs can be modulated by physical or biochemical stimuli. My research scope has covered two areas:

    1. Using proteomic approach to globally demonstrate how protein composition in FAs is modulated by physical or biochemical stimuli.

    2. Using microscope and biochemical technologies to demonstrate the molecular mechanism of how FA-associated proteins regulate cellular events, including cell migration, proliferation, differentiation or death.


    • Hsiao CT, Cheng HW, Huang CM, Lee HR, Ou MH, Huang JR, Khoo KH, Yu HW, Chen YQ, Wang YK, Chiou A, Kuo JC*. Fibronectin in cell adhesion and migration via N-glycosylation. Oncotarget 2017. (in revision)


    • Chuang TY, Cheng AJ, Lan TY, Huang IH, Shiau CW, Hsu CL, Liu YW, Tseng PH, Chang ZF, Kuo JC*. Suppression of LPS-induced inflammatory responses by the hydroxyl groups of dexamethasone. Oncotarget 2017. (accepted)


    • Chakraborty S, Ou MH,Kuo JC, Chiou A. A comparative study of metabolic state of stem cells during osteogenic and adipogenic differentiations via fluorescence lifetime imaging microscopy. Proc. of SPIE Vol. 10024:100243Y. 2016.


    • Wu TH, Kuo YY, Lee HH, Kuo JC, Ou MH, Chang ZF. Epigenetic repression of ribosomal RNA transcription by ROCK-dependent aberrant cytoskeletal organization. Sci. Rep. 6:28685. 2016.


    • Wu JC, Chen YC, Chen YQ Chen, Chiou A, Kuo JC*. Focal adhesion kinase-dependent focal adhesion recruitment of SH2 domains directs SRC into focal adhesions to regulate cell adhesion and migration. Scientific Report. 5:18476 . 2015.


    • Wang HL, Yang CH, Lee HH, Kuo JC, Hur SS, Chien S, Lee OK, Hung SC, Chang ZF. Dexamethasone-induced cellular tension requires SGK1-stimulated Sec5/GEF-H1 interaction. J Cell Sci. pii: jcs.169961. [Epub ahead of print]. 2015.


    • Yu HW, Chen YQ, Huang CM, Liu CY, Chiou A, Wang YK, Tang MJ, Kuo JC*. b-PIX controls intracellular viscoelasticity to regulate lung cancer cell migration. J Cell Mol Med. 19(5): 934-947. 2015.


    • Huang IH, Hsiao CT, Shen RF, Liu CY, Wang YK, Chen YC, del Alamo JC, Chang ZF, Tang MJ, Khoo KH, Kuo JC*.GEF-H1 controls focal adhesion signaling that regulates mesenchymal stem cell lineage commitment. J Cell Sci.127:4186-4200. 2014.* This paper was selected to be the main Journal of Cell Science October cover.


    • Kuo JC. Focal adhesions function as a mechanosensor. Progress in Molecular Biology and Translational Science.126:55-73. 2014.


    • Yuan WC, Lee YR, Lin SY, Chang LY, Tan YP, Hung CC, Kuo JC, Liu CH, Lin MY, Xu M, Chen ZJ, Chen RH. K33-Linked Polyubiquitination of Coronin 7 by Cul3-KLHL20 Ubiquitin E3 Ligase Regulates Protein Trafficking. Mol. Cell pii: S1097-2765(14)00268-8. 2014.


    • Kuo JC. Mechanotransduction at focal adhesions: integrating cytoskeletal mechanics in migrating cells. J Cell Mol Med.17(6):704-712. 2013.


    • Chen DY, Li MY, Wu SY, Lin YL, Tsai SP, Lai PL, Lin YT, Kuo JC, Meng TC, Chen GC. The Bro1 domain-containing Myopic/HDPTP coordinates with Rab4 to regulate cell adhesion and migration. J Cell Sci. 125(pt20): 4841-52. 2012.


    • Kuo JC, Han X, Yates JR, Waterman CM. Isolation of focal adhesion proteins for biochemical and proteomic analysis. Method Mol. Biology (Integrin and Cell Adhesion Molecules). 757:297-323. 2012.


    • Valdes JL, Tang J, Mcdermott MI, Kuo JC, Zimmerman SP, Wincovitch SM, Waterman CM, Milgram SL, Playford MP. Sorting Nexin 27 mediates PDZ-directed targeting of a β-Pix-Git complex to focal adhesions. J. Biol. Chem. 2011. (in press).


    • Kuo JC, Han X, Hsiao CT, Yates JR, Waterman CM. Analysis of the myosinII-responsive focal adhesion proteome reveals a role for β-Pix in negative regulation of focal adhesion maturation. Nat. Cell Biol. 13(4):383-93. 2011.

      *This paper was selected to be the main Nature Cell Biology April cover, and was the subject of the news and views in Nature Cell Biology (Joan S. Brugge, 13: 344, 2011) and a research highlight in Nature Reviews Molecular Cell Biology (Katrin Legg, 12:278, 2011).

      Myosin II-Responsive Focal Adhesion Proteome:


    • Wang WJ, Kuo JC, Ku W, Lee YR, Lin FC, Chang YL, Lin YM, Chen CH, Huang YP, Chiang MJ, Yeh SW, Wu PR, Shen CH, Wu CT, and Chen RH. The tumor suppressor DAPK is reciprocally regulated by tyrosine kinase Src and phosphatase LAR. Mol. Cell. 27(5):701-16. 2007.


    • Kuo JC, Wang WJ, Yao CC, Wu PR, and Chen RH. The tumor suppressor DAPK inhibits cell motility by blocking integrin-mediated polarity pathway. J Cell Biol. 172(4): 619-31. 2006.


    • Chen RH, Wang WJ, and Kuo JC. The tumor suppressor DAP-kinase links cell adhesion and cytoskeleton reorganization to cell death regulation. J Biomed Sci. 3: 1-7. 2006.


    • Chen CH, Wang WJ, Kuo JC, Tsai HC, Lin JR, Chang ZF, Chen RH. Bidirectional signals transduced by DAPK-ERK interaction promote the apoptotic effect of DAPK. EMBO J. 24: 294-304. 2005.


    • Kuo JC, Lin JR, Staddon JM, Hosoya H, Chen RH. Uncoordinated regulation of stress fibers and focal adhesions by DAP kinase. J Cell Sci. 116: 4777-4790. 2003.


    • Wang WJ, Kuo JC, Yao CC, Chen RH. DAP-kinase induces apoptosis by suppressing integrin activity and disrupting matrix survival signals. J Cell Biol. 159: 169-79. 2002.

    Voice Play