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張智芬

張智芬

研究領域--核酸代謝與DNA修復,訊息與細胞調控

1. Nucleotide metabolism in DNA damage

The molecular mechanism regulating dTTP synthesis is coordinated with DNA damage signal for repair. We focus on two research aspects: (i) the mechanism for DNA damage site-specific production of dTTP, (ii) design of small molecules targeting dTTP formation for specific intervention of DNA repair in malignant tumor cells.

 

2. Assessment of the Rho-ROCK pathway in differentiation and stress response

RhoA/ROCK-mediated contractility is critical for many cellular processes. Currently, we are interested in understanding how RhoA-ROCK pathway regulates gene expression in osteoblast committment of mesenchymal stem cell and centrosomal overduplication in response to DNA damage.

研究著作

Ning Tsao, Ming-Hsiang Lee, Wei Zhang, Yung-Chi Cheng, and Zee-Fen Chang*. The contribution of CMP kinase to the efficiency of DNA repair. Cell Cycle 14:3, 1--10; December 1, 2014

 

Ming-Hsiang Lee, Liya Wang and Zee-Fen Chang*. The contribution of mitochondrial thymidylate synthesis in preventing the nuclear genome stress. Nucleic Acids Research, 2014,1-13 doi:10.1093/nar/gku152

 

Tien SC and Chang ZF*. Oncogenic Shp2 disturbs microtubule regulation to cause HDAC6-dependent ERK hyperactivation. Oncogene. 2013 Jun 17. doi: 10.1038/onc.2013.241. [Epub ahead of print]

 

Pan YR, Cho KH, Lee HH, Chang ZF*, Chen HC. Protein tyrosine phosphatase SHP2 suppresses podosome rosette formation in Src-transformed fibroblasts. J Cell Sci. 2013 Jan 15;126(Pt 2):657-66. doi: 10.1242/jcs.116624.

 

Hsiang-Hao Chuang, Shao-Wei Liang, Zee-Fen Chang* and Hsiao-Hui Lee*. Ser1333 phosphorylation indicates ROCKI activation. Journal of Biomedical Science 2013, 20:83

 

Chuang HH, Yang CH, Tsay YG, Hsu CY, Tseng LM, Chang ZF*, Lee HH*. ROCKII serine 1366 phosphorylation reflects the activation status. Biochemical Journal. 443: p145-151, 2012. (*: corresponding authors)

 

Hu, CM, Yeh, MT, Tsao, N, Chen, CW, Gao, QZ, Chang, CY, Lee, MH, Fang, JM, Sheu, SY, Lin, CJ, Tseng, MC, Chen, YJ, Chang, ZF*. Tumor Cells Require Thymidylate Kinase to Prevent dUTP Incorporation during DNA Repair. Cancer cell, 22(1) 36 - 50, 2012.

 

Lee HH, Tien SC, Jou TS, Chang YC, Jhong JG, and Chang ZF*. c-Src-dependent phosphorylation of ROCK participates in regulation of focal adhesion dynamics. J Cell Science. 123:3368-3377, 2010.

 

Chen YL, Eriksson S, Chang ZF*. The regulation and functional contribution of thymidine kinase 1 in repair of DNA damage. J Biol Chem. 285:27327-27335, 2010.

 

Hu CM, Chang ZF*. A bioluminescent method for measuring thymidylate kinase activity suitable for high-throughput screening of inhibitor. Anal Biochem. 2009 Dec 5. doi:10.1016/j.ab.2009.12.001

 

Chang YC, Tien SC, Tien HF, Zhang H, Bokoch GM and Chang ZF*. p210Bcr_Abl desensitizes Cdc42 GTPase signaling for SDF-1a-directed migration in chronic myeloid leukemia cells. Oncogene. 28:4105-15, 2009.

 

Lee HH and Chang ZF*. Regulation of RhoA-dependent ROCK activation by Shp2. J. Cell Biol. 181:999-1012, 2008.

 

Chen YL, Lin DW, and Chang ZF*. Identification of a Putative Human Mitochondrial Thymidine Monophosphate Kinase Associated with Monocytic/Macrophage Terminal Differentiation. Genes to Cells. 13: 679-89, 2008.

 

Hu CM and Chang ZF*. Synthetic lethality by lentiviral short hairpin RNA silencing of thymidylate kinase and doxorubicin in colon cancer cells regardless of the p53 status. Cancer Res. 68:2831-40, 2008.

 

Chang YC, Nalbant P, Birkenfeld J, Chang ZF*, Bokoch, GM. GEF-H1 couples nocodazole-induced microtubule disassembly to Cell Contractility via RhoA. Mol Biol Cell. 19:2147-53, 2008.

 

Chou WL, Chuang LM, Chou CC, Wang AHJ, Lawson JA, FitzGerald, GA and Chang ZF*. Identification of a novel prostaglandin reductase reveals the involvement of prostaglandin E2 catabolism in regulation of peroxisome proliferator-activated receptor gamma activation. J Biol Chem. 282:18162-72, 2007.

 

Kuo YY, and Chang ZF*. GATA-1 and Gfi-1B interplay to regulate Bcl-xL transcription. Mol Cell Biol. 27:4261-72, 2007.

 

Ke PY, Hu CM., Chang YC, and Chang ZF*. Hiding human thymidine kinase 1 from APC/C-mediated destruction by thymidine binding. FASEB J. 21:2176-84, 2007.

 

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